簡介

研究室簡介
Introduction to the CCV Laboratory 

緣起

　　民國89年12月，林茂村教授在詹啟賢前院長邀請下，從國立陽明大學借調至奇美醫院籌組研究室，林教授領軍陽明大學博士生團隊，積極建立軟硬體設施，隔年3月腦心血管研究室正式成立。初期為了讓臨床醫師可以了解基礎研究工作的重要性，每個月舉行實驗室會報(Laboratory meeting)，內容以國際期刊文獻導讀及實驗進度報告為主，並從中提出解決疾病問題的相關實驗設計，結合基礎與臨床工作者的綜合討論，引起醫療同仁們熱烈的回響，激發出許多創意思維，這場【From bench to beside】討論會至今超過20年。林教授所指導的研發團隊在創新沃土中開枝散葉，多位博士生及臨床醫師，已能自立研究室，延續著生命醫學研究量能。

Origin

　　Professor Mao-Tsun Lin initiated the Cerebrocardiovascular Laboratory (CCV Lab) at the end of 2000, invited by former Dean Chi-Hsien Chan, leading a team of doctoral students from National Yang-Ming University to develop the necessary infrastructure for basic research. The CCV Lab was officially established in March of the following year. In 2016, his student, Professor Ching-Ping Chang, took over the CCV Lab. CCV Lab comprises nine full-time research assistants, one advisor (Prof. Mao-Tsun Lin), and a principal investigator (Prof. Ching-Ping Chang). We are committed to promoting innovative R&D and finding solutions to disease problems. We are dedicated to providing our doctors and colleagues with robust experimental design services and infrastructure support. We aim to facilitate the research journey from conception to dissemination, ensuring that each discovery has the most significant impact.

　　In the early period of the CCV Lab establishment, we held monthly laboratory meetings to help clinical physicians understand the importance of basic science research work. These meetings primarily focused on literature reviews of international journals and progress reports on experiments. They also included the design of experiments relevant to solving disease problems, combining comprehensive discussions between basic scientists and clinical physicians. These discussions generated enthusiastic responses from medical colleagues and sparked numerous creative ideas. The "From Bench to Bedside" conversations have continued for over 20 years. Under the guidance of Professor Lin, the research and development team has flourished in this innovative environment. Many PhD students and clinical physicians have established their labs, continuing the momentum of biomedical research.

紮實基礎研究，奇美學術深耕

　　過去二十幾年，在院方大型經費挹注及林茂村教授及張菁萍教授帶領下，腦心血管研究室陸續建構了模擬臨床疾病之動物與細胞模式，包括:　腦中風、熱中風、頭部創傷、脊髓損傷、阿茲海默症、出血性休克、高山症、心肌梗塞、肝臟缺血性損傷、視網膜損傷、新生幼鼠腦缺氧缺血性損傷、新生鼠壞死性腸炎、肺損傷、牙周病及骨折等。建立研究平台，並開發從基因體到蛋白質體相關分析技術，提供了各科醫事人員進行研發及論文發表，學術表現成就斐然，包括：(1)發表SCI文章超過500篇；(2)協助教職申請；(3)指導碩博士學位論文；(4)協助爭取國科會計畫經費補助超過50件；(5)獲得台灣及美國發明專利超過10件。林教授與張教授這二十年來往下紮根，向上結果，已為奇美醫院的研究發展建立堅強的磐石。

　　研究工作的推動，仰賴著由一群知識份子所組成的堅強團隊陣容，包括了何孟聰、唐聆祐、趙苾聿、陳子豪、簡嵐翔、熊建瑋、趙思涵、黃永欣、張育瑋、柳文濱、平祖妮、馬瑞悌、王鐘漢、許瑜倢、廖禹涵、王家卉、王育純、陳思瑜、黃健峻、陳宥婕、塗惠媛、郭庭維、劉宇帆、楊仲智、林楠凱、陳思婷、陳佳瑩、許家諆、田如伶、姚雲欽、許瓊月、邱晏婷等研究助理們，協助所有課題的執行，能有此番研究成果，他們功不可沒。

　　實驗室重視學術倫理，為強化研究規範，建立了(1)實驗日誌撰寫準則，(2)實驗標準作業程序手冊，(3)儀器設備財產管理清冊，(4)藥品、試劑、抗體列管使用清冊，及(5)實驗室物質安全資料表。各項軟硬體設施定期盤點及校正，研究室工作人員詳實紀錄科學研究歷程，並恪守分析方法學SOP，降低人為誤差並提高實驗再現性。這些規範將會永存於奇美醫院腦心血管研究室，也將提供後代綿延不絕的學子們最佳科學訓練的典範。

Solid foundational research and deep cultivation of academics at Chi Mei Medical Center

　　In the past two decades, with substantial funding from the Chi Mei Medical Center and the leadership of Professor Mao-Tsuan Lin and Prof. Ching-Ping Chang, the CCV Laboratory has progressively developed animal and cell models to simulate clinical diseases. These include models for cerebral stroke, heat stroke, traumatic brain injury, spinal cord injury, Alzheimer's disease, hemorrhagic shock, altitude sickness, myocardial infarction, ischemic liver injury, retinal injury, neonatal hypoxia & ischemia injury, necrotizing enterocolitis, lung injury,periodontitis, and bone fracture. They have established research platforms and developed related analytical techniques from genomics to proteomics & metabolomics, from single-cell sequencing to histology, providing medical professionals from various specialties with the tools for research and publication. The academic achievements have been outstanding, including:

　　The advancement of our research work relies on a solid team of intellectuals, including Meng-Tsung Ho, Ling-Yu Tang, Pi-Yu Chao, Tzu-Hao Chen, Lan-Hsiang Chien, Chien-Wei Hsiung, Ssu-Han Chao,Yung-Xin Huang,Yu-Wei Chang,Wen-Pin Liu,Tsu-Ni Ping, Jui-Ti Ma, Chung-Han Wang, Yu-Chih Hsu, Yu-Han Liao, Jia-Hui Wang, Yu-Chun Wang, Ssu-Yu Chen,Chien-Chun Huang, Yu-Chieh Chen, Hui-Yuan Tu, Ting-Wei Kuo, Yu-Fan Liu, Chung-Zhing Yang, Nan-Kai Lin, Ssu-Ting Chen, Chia-Ying Chen, Jia-Qi Hsu, Ru-Ling Tian, Yun-Qin Yao, Qiong-Yue Hsu, and Yan-Ting Chiu. ​​These research assistants have contributed significantly to the execution of all projects. The research achievements would not have been possible without their invaluable contributions. 

　　The CCV laboratory places great emphasis on academic ethics. To strengthen research standards, we have established the following: (1) guidelines for writing an experimental notebook, (2) a manual of standard operating procedures for experiments, (3) an inventory for the management of instruments and equipment, (4) a register for the use of drugs, reagents, and antibodies, and (5) material safety data sheets for the laboratory. All software and hardware facilities are regularly inventoried and calibrated. Laboratory staff meticulously record the scientific research process and strictly adhere to the SOP for analytical methods, reducing human error and increasing the reproducibility of experiments. These standards will be permanently upheld in the Chi Mei Medical Center CCV Laboratory and will provide a model of the best scientific training for future generations of students. 

推動研發創新， 強化國際交流

　　林教授重視基礎知識的建立，並鼓勵獨立自主思考與挑戰權威、重視創意，舉凡臨床醫師提出待解決的課題，皆要求醫師們回歸到基本面，從閱讀做起，收集與分析文獻，希冀研究假說的成立，必要能為現今知識提供更進一步解釋。進一步協助動物或細胞模型建立，引用最新分子生物技術，若有不足則著手開發研究設備。以頭部外傷動物模式為例，採用gene array方式篩選出可供預後評估的分子標記，並自行研發設計最適化運動復健平台 (已獲得發明專利)。鼓勵、支持創新研發，也將資源分享給海內外合作醫院及學校，輔導研究工作的進行，包括:台北醫學大學、馬偕醫院及大學、雙和醫院、陽明大學、成功大學、南臺科技大學、嘉南藥理科技大學、勤益科技大學、美國哈佛大學、日本早稻田大學、澳洲University of Wollongong、北京軍事醫學科學院、北京首都醫科大學、山東濟南軍區總醫院、廣州軍區總醫院等單位。 此外，林教授肩負海峽兩岸學術交流之重任，擔任台灣地區召集人，帶領醫事人員參與兩岸學術交流及國際研討會，並籌劃國際型研討會在醫院舉行。已有24年歷史的海峽兩岸心血管科學研討會，未來也將交由林宏榮院長及張菁萍教授延續下去。

Promote research and innovation and strengthen international exchange

　　Professor Mao-Tsun Lin emphasizes establishing fundamental knowledge, encouraging independent thinking, challenging authority, and valuing creativity. When clinical physicians propose resolving issues, they must return to the basics, starting with reading, collecting, and analyzing literature, hoping that forming research hypotheses will provide further explanations for current knowledge. Additionally, assistance is provided in establishing animal or cell models and utilizing the latest molecular biology techniques. If necessary, research equipment is developed. For example, gene array methods were used in the animal model of traumatic brain injury to screen for molecular markers for prognostic evaluation, and an optimized motor rehabilitation platform was self-developed and patented. 

　　Innovation and research development are encouraged and supported, and resources are shared with collaborative hospitals and schools domestically and internationally. These include Taipei Medical University, Mackay Memorial Hospital and University, Shuang Ho Hospital, National Yang-Ming University, National Cheng Kung University, Southern Taiwan University of Science and Technology, Chia Nan University of Pharmacy and Science, National Chin-Yi University of Technology, Harvard University, Waseda University, University of Wollongong, Academy of Military Medical Sciences in Beijing, Capital Medical University in Beijing, Jinan Military General Hospital in Shandong, and Guangzhou Military General Hospital.​​

　　Furthermore, Professor Lin is responsible for academic communications across the strait, serving as the convener for Taiwan, leading scientists and physicians to participate in academic exchanges and international conferences, and organizing international symposiums at the hospital. The Cross-Strait Cardiovascular Science Conference, with a 24-year history, will be continued by Dean Hong-Rong Lin and Professor Ching-Ping Chang in the future. 

帶領產學研合作，培育生醫人才

　　研究團隊過去成功建立多種神經損傷動物模式，各項神經再生、運動及認知功能評估研究技術穩定成熟，將此研發量能實務應用於輔導國內生技公司，進行產品開發製成設計、臨床前實驗設計、細胞品質與功能分析、實驗標準作業程序建立。關於幹細胞相關研發成果豐碩，已至少30篇論文發表於國際期刊。此外，協助台灣生技產業人才培育，也是研究室的重點工作之一。研究室每年舉辦生物技術研習，協助南臺科技大學師生高階儀器 (例如:流式細胞儀、螢光顯微鏡) 操作及細胞、動物實驗技術訓練，至今已超過300人次。腦心血管研究室每年提供暑期實習機會，指導來自美國、台灣的高中生、大學生進行專題實驗。值得一提的是，與生技公司合作案中，提供5位大專生實習機會，並每月提供獎助學金，讓學生安心在醫院實習，並藉由計畫執行與產業界連結，加強學習延續性，順利接軌業界，降低學用落差。此【醫院-學校-產業】三方合作，樹立台灣產學研合作之典範。 

　　時間的巨輪不斷向前，這二十年來，林教授持續推動學術研究的多元價值，從未停歇。研發成果質量並重，充分發揮科研資源整合效益，推動前瞻科技研究與創新，培育醫學研究人才，提升醫院整體科技研究水準。林教授所建立的目標明確，也讓繼任者得以順利承接醫院賦予的任務及挑戰。 

Lead industry-academia-research collaboration and cultivate biomedical talent

　　The research team has successfully established various animal models of neural injury in the past, with stable and mature techniques for assessing neural regeneration and motor and cognitive functions. These research capabilities have been practically applied in assisting Taiwan biotech companies with product development, process design, preclinical experiment design, cell quality, function analysis, and establishing standard operating procedures for experiments. The research achievements related to stem cells are substantial, with at least 30 papers published in international journals. 

　　In addition, one of the key tasks of CCV Laboratory is cultivating talent for the biotech industry in Taiwan. The laboratory organizes biotechnology workshops annually, assisting faculty and students from Southern Taiwan University of Science and Technology in operating advanced instruments (such as flow cytometers and fluorescence microscopes) and training for cell and animal experiment techniques, benefiting over 300 individuals. The CCV Laboratory offers summer internship opportunities annually, guiding high school and undergraduate students from the United States and Taiwan in conducting research projects. 

　　In collaboration with biotech companies, the lab provides internship opportunities for five college students, offering monthly scholarships to ensure they can intern at the hospital with peace of mind. Through the execution of projects and connections with the industry, this initiative enhances the continuity of learning, facilitates smooth transitions to the industry, and reduces the gap between academia and practical application. This "Hospital-School-Industry" tripartite collaboration sets a model for industry-academia-research cooperation in Taiwan. 

　　The wheel of time keeps moving forward, and over the past twenty years, Professor Mao-Tsun Lin has continuously promoted the diverse values of academic research, never pausing. The research and development achievements balance quality and quantity, fully leveraging the benefits of integrating research resources, advancing cutting-edge technology research and innovation, cultivating medical research talent, and enhancing the overall scientific research standards of the hospital. The precise objectives established by Professor Lin have also enabled his successors to undertake the tasks and challenges assigned by the Chi Mei Medical Center. 

世代傳承，緣起不滅

　　2016年8月1日，林教授正式將腦心血管研究室交棒給他的學生張菁萍教授，完成了世代傳承，研究室過去所奠定的成果，也將在張教授手中延續下去。她，師承林茂村教授已近26年，受到林教授重點栽培，其在學期間與大陸、香港、台灣的博士生競逐科學獎，獲得第一屆海峽兩岸心血管科學研討會傑出青年研究獎，畢業後至南臺科技大學生物科技系擔任助理教授，教學認真負責並多次獲得優良教師獎，深受學生愛戴。研究表現傑出，執行多年期科技部計畫及獲得多次傑出優秀人才獎勵，並熱衷於設計研發動物生理監測設備且獲多項台灣及美國發明專利，在39歲即升等教育部定教授，研究成果受到國際期刊重視，多次獲邀國際研討會擔任講者及撰寫綜合評論性文章，根據2021 Jan 21於Mil Med Res. 8(1):5文獻計量分析指出張教授在熱中暑領域文章發表與引用數量為世界排名第五。她的研究熱情與研究量能將帶領研究團隊繼續前進。 

　　2016年，腦心血管研究室完成了世代交替，科學精神也將永續。 

In generational inheritance, the origin never fades

　　On August 1, 2016, Professor Lin officially handed over the Cerebrocardiovascular Laboratory to his student, Professor Ching-Ping Chang, completing the generational transition. The achievements established by the lab will continue under Professor Chang's leadership. She has studied under Professor Mao-Tsun Lin for over 26 years, receiving focused mentorship. During her studies, she competed with doctoral students from mainland China, Hong Kong, and Taiwan for science awards, winning the Outstanding Young Researcher Award at the first Cross-Strait Cardiovascular Science Symposium. After graduation, she served as an assistant professor in the Department of Biotechnology at Southern Taiwan University of Science and Technology. Her dedicated teaching earned her multiple Excellent Teacher Awards and the affection of her students. 

　　Prof. Chang has outstanding research performance, has executed multi-year Ministry of Science and Technology projects, and has received multiple excellent talent grant support. She is also keen on designing and developing animal physiological monitoring equipment and has obtained many invention patents in Taiwan and the United States. She was promoted to a full professor appointed by the Ministry of Education at 39. International journals have valued her research results, and she has been invited to serve as a speaker at international seminars and has written comprehensive review articles many times. According to the bibliometric analysis of Mil Med Res. 8(1):5 on January 21, 2021, Professor Chang has made outstanding achievements in the heat stroke field. The number of articles published and cited ranks fifth in the world. Her research enthusiasm and research volume will lead the research team to move forward. 

　　In 2016, the CCV Lab completed its generational transition, ensuring the perpetuation of the scientific spirit.